Anticorpos plásticos em papel condutor para deteção de biomoléculas

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Anticorpos plásticos em papel condutor para deteção de biomoléculas

Sexta, 01.07.2016

Neste trabalho apresenta-se uma forma simples e barata de produzir um dispositivo sensível a uma biomolécula. O dispositivo consiste em papel hidrofobizado com parafina, recoberto com uma tinta de grafite caseira e com um material sensor (anticorpo plástico) obtido localmente, por aplicação de um estímulo elétrico.

A biomolécula alvo neste estudo foi a carnitina, que é um potencial biomarcador no cancro do ovário, mas o conceito de dispositivo aqui apresentado pode ser alargado a outras biomoléculas, incluindo outros biomarcadores do cancro.

 

Autores e Afiliações:

Ana Patrícia Moreira Tavares, Nádia Silva Ferreira, Liliana Adelina Afonso Novo de Almeida Truta, Maria Goreti Ferreira Sales

BioMark-CINTESIS/ISEP, Instituto Superior de Engenharia do Porto, do Instituto Politécnico do Porto, Portugal

 

Abstract:

This work reports a novel way of producing a sensing film on an inexpensive substrate support, designed for electrical transduction of an intended biomolecule. The support uses cellulose paper as substrate, made hydrophobic with solid wax and covered by a home-made conductive ink having graphite as core material. The hydrophobicity of the paper was confirmed by contact angle measurements and the conductive ink composition was optimized with regard to its adhesion, conductivity, and thermal stability. This support was further modified targeting its application in quantitative analysis. Carnitine (CRT) was selected as target compound, a cancer biomarker. The recognition material consisted of an antibody-like receptor film for CRT, tailored on the support and prepared by electrically-sustained polymerization of 3,4-ethylenedioxythiophene (EDOT) or dodecylbenzenesulfonic acid (DBS). Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy analysis confirmed the presence of the polymeric film on the support, and the performance of the devices was extensively evaluated with regard to linear response ranges, selectivity, applicability, and reusability. Overall, the paper-based sensors offer simplicity of fabrication, low cost and excellent reusability features. The design could also be extended to other applications in electrical-based approaches to be used in point-of-care (POC).

 

Revista: Scientific Reports

 

Link: http://www.nature.com/articles/srep26132