Volatiloma urinário para o diagnóstico do cancro da próstata

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Volatiloma urinário para o diagnóstico do cancro da próstata

Terça, 17.12.2019

A falta de um biomarcardor sensível e específico para a deteção precoce do cancro da próstata constitui um obstáculo ao tratamento eficaz destes doentes. Este trabalho pretendeu estudar o perfil metabólico volátil de urinas de doentes com cancro da próstata (n=58) e de indivíduos controlo (sem cancro, n=60), com o objetivo de identificar um potencial painel de biomarcadores para a deteção não invasiva do cancro da próstata. Os resultados obtidos permitiram estabelecer um painel de biomarcadores constituído por 6 compostos voláteis, que mostrou ser capaz de identificar este tipo de cancro com uma sensibilidade, especificidade e precisão de 89%, 83% e 86%, respetivamente. Esta metodologia mostrou ter uma melhor sensibilidade e precisão que o antigénio específico da próstata (PSA), que é o biomarcador mais frequentemente utilizado no rastreio do cancro da próstata na prática clínica.

 

Autores e Afiliações:

Lima AR1, Pinto J2, Azevedo AI2, Barros-Silva D3, Jerónimo C3,4, Henrique R3,4,5, de Lourdes Bastos M2, Guedes de Pinho P6, Carvalho M7,8

1 UCIBIO/REQUIMTE, Department of Biological Sciences, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, Porto, Portugal. 

2>UCIBIO/REQUIMTE, Department of Biological Sciences, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, Porto, Portugal.

3Cancer Biology Epigenetics Group, Research Center (CI-IPOP) Portuguese Oncology Institute of Porto (IPO Porto), Porto, Portugal.

4 Department of Pathology and Molecular Immunology-Biomedical Sciences Institute (ICBAS), University of Porto, Porto, Portugal.

Department of Pathology, Portuguese Oncology Institute of Porto (IPO Porto), Porto, Portugal.

6 UCIBIO/REQUIMTE, Department of Biological Sciences, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, Porto, Portugal.

7UCIBIO/REQUIMTE, Department of Biological Sciences, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, Porto, Portugal.

UFP Energy, Environment and Health Research Unit (FP-ENAS), University Fernando Pessoa, Porto, Portugal.

 

Abstract:

Background: The lack of sensitive and specific biomarkers for early detection of prostate cancer (PCa) is a major hurdle to improve patient management. Methods: A metabolomics approach based on GC-MS was used to investigate the performance of volatile organic compounds (VOCs) in general and, more specifically, volatile carbonyl compounds (VCCs) present in urine as potential markers for PCa detection. Results: Results showed that PCa patients (n=40) can be differentiated from cancer-free subjects (n=42) based on their urinary volatile profile in both VOCs and VCCs models, unveiling significant differences in the levels of several metabolites. The models constructed were further validated using an external validation set (n=18 PCa and n=18 controls) to evaluate sensitivity, specificity and accuracy of the urinary volatile profile to discriminate PCa from controls. The VOCs model disclosed 78% sensitivity, 94% specificity and 86% accuracy, whereas the VCCs model achieved the same sensitivity, a specificity of 100% and an accuracy of 89%. Our findings unveil a panel of 6 volatile compounds significantly altered in PCa patients’ urine samples that was able to identify PCa, with a sensitivity of 89%, specificity of 83%, and accuracy of 86%.Conclusions: It is disclosed a biomarker panel with potential to be used as a non-invasive diagnostic tool for PCa.

 

Revista: British Journal of Cancer

 

 

Link: https://www.ncbi.nlm.nih.gov/pubmed/31588123