Expression of CD44 and CD35 during normal and myelodysplastic erythropoiesis

Authors and Affiliations:

Paula Laranjeira^a*, Raquel Rodrigues^b,c*, Tiago Carvalheiro ^a, Conceição Constanço^c, Helena Vitória^c, Sergio Matarraz^d, Hélder Trindade^a, Alberto Órfão ^d and Artur Paiva PhD^a,b

^a Centro do Sangue e da Transplantação de Coimbra, Instituto Português do Sangue e da Transplantação, Quinta da Vinha Moura, São Martinho do Bispo, 3041-861 Coimbra, Portugal

^b Escola Superior de Tecnologia da Saúde de Coimbra, Rua 5 de Outubro, 3046-854 Coimbra, Portugal

^c Centro Hospitalar Tondela-Viseu, EPE, Av. Rei D. Duarte, 2504-509 Viseu, Portugal

^d Centro de Investigación del Cáncer (Instituto de Biología Molecular y Celular del Cáncer; CSIC-USAL), Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain

* Estas autoras contribuíram igualmente na realização do trabalho e devem ser consideradas co-primeiras autoras

Abstract:

Erythroid dysplasia is a common feature of myelodysplastic syndromes (MDS). Currently available information about the immunophenotypic features of normal and dysplastic erythropoiesis is scarce and restricted to relatively few markers. Here we studied the expression of CD117, CD35 and CD44 throughout the normal (n = 16) and dysplastic (n = 48) bone marrow erythroid maturation. CD35 emerged as an early marker of CD34+ erythroid-committed precursors, which is expressed before CD105 and remains positive thereafter. MDS patients (with and without morphologic dyserythropoiesis) displayed overall increased expression of CD44, associated with slight alterations on CD35 expression, suggesting that phenotypic alterations in MDS may precede morphologic dysplasia. In turn, MDS patients with anemia showed increased expression of CD117.

Highlights

• CD35 is an early marker of CD34+ erythroid-committed precursors.

• CD35 is expressed before CD105 in CD34+ erythroid-committed precursors.

• MDS patients display overall increased expression of CD44.

• Our data suggest that phenotypic alterations in MDS precede morphologic dysplasia.

• MDS patients with anemia show increased expression of CD117 vs. MDS without anemia.

Journal: Leukemia Research

Link: http://www.lrjournal.com/article/S0145-2126(14)00404-4/abstract